Erscheinungsdatum: 15.12.2015, Medium: Buch, Einband: Gebunden, Titel: Cancer Gene Profiling, Titelzusatz: Methods and Protocols, Auflage: 2. Auflage von 2015 // 2nd ed. 2016, Redaktion: Grützmann, Robert // Pilarsky, Christian, Verlag: Springer New York // Springer US, Sprache: Englisch, Schlagworte: Erblehre // Genetik // Krebs // Krankheit // Tumor // Medizin, Rubrik: Allgemeinmedizin // Diagnostik, Therapie, Seiten: 268, Informationen: HC runder Rücken kaschiert, Gewicht: 683 gr, Verkäufer: averdo
MicroRNAs (miRNAs) are endogenous, small non-coding RNA molecules that can play important regulatory role in animals and plants by targeting specific mRNA, directly degrading it and/or preventing its translation into protein. miRNAs have been shown to play crucial role in numerous biological processes, cellular pathways and networks. The impaired miRNA function results in aberrant gene expression that promotes abnormal cell growth and differentiation. miRNAs are located at fragile sites or cancer associated regions of the genome, they act as either tumor suppressor or tumor promoting (oncomiRs) miRNAs, thereby, involving in tumorigenesis and tumor progression. miRNAs are also found to have immense role in aging and its associated events in cells. Given the involvement of miRNAs in cancer and aging, they have become an area of intense research focus in recent years. The quantitative and qualitative assessment of miRNAs expression have shown remarkable changes in expression profiles in various diseases other than in cancer and aged states. Thus, profiling of miRNA expression can be an important tool for diagnostics and treatment of diseases including disorders like cancer and aging.
Dogs and cats are the most beloved creatures among the pet animal species therefore, animal lovers are more anxious about their companion animal's health and husbandry techniques. Animal cancer incidence rate is increasing due to less rigorous managerial efforts, fewer animal health care facilities and economic constraints. The current study was planned to analyze the expression profiling of Hspb1 & Tp53 genes through RT-qPCR in seven different types of cancers in these two valued pet species in Pakistan. Secondly, characterization of the aforementioned genes was also performed to ascertain diseased associated mutations/SNP, which can served as diagnosis and prognosis markers for these malignancies. Thirdly, how these novel signature cancer associated mutations are associated with particular tumor type by population screening of 208 cats from different seven countries of the world. This book will make available the gene expression profiles and cancer associated mutations along with population screening data in relation to seven different types of cancer, which is a substantial addition to the existing information of animal genetic resource (AnGR) in Pakistan.
Breast Cancer is the most common tumor in women and the second leading cause of cancer deaths worldwide. Due to breakthroughs in gene profiling, the knowledge of the pathophysiology of the mammary gland had greatly increased over the last decade. In Breast Cancer in the Post Genomic Era, Antonio Giordano, Nicola Normanno, and a panel of international authorities in their field provide a comprehensive approach to the biology, diagnosis, prevention, and treatment of human breast carcinoma.The book provides a comprehensive approach to breast cancer, describing the use of gene profiling techniques to distinguish specific features of individual carcinomas, as well as emerging novel therapeutic approaches to treatment. Additional chapters cover the use of transgenic mice to model human breast cancer and the role of the EGF-CFC family in mammary gland development and neoplasia. Breast Cancer in the Post Genomic-Era succeeds in looking at breast cancer pathogenesis, diagnosis, and treatment under a more comprehensive light, and is a valuable resource for any Radiation or Surgical Oncologist, Cancer Biologist or Pathologist.
Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity, yet many questions still remain. In MicroRNA Protocols, Second Edition , experts in the field provide up-to-date coverage of this diverse area of study. The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls.Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.
Proteases decisively contribute to cancer development and promotion by regulating the activities of growth factors/cytokines and signaling receptors, as well as the composition of the extracellular matrix, thereby suppressing cell death pathways and activating cell survival pathways. In Proteases and Cancer: Methods and Protocols , expert researchers bring together a wide range of current, complimentary techniques that have been developed for the specific detection and analysis of proteases and their activities in cancer biology. The volume covers vital topics including the application of proteomics technologies for the detection of protease expression in tumors, imaging proteases by activity profiling, proteomics technologies for the identification of biological substrates, detection of cell surface proteolysis, imaging of protease activity, the use of transgenic mice to determine protease function in tumor initiation and progression, and the development of anti-protease therapies for cancer. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy to use, Proteases and Cancer: Methods and Protocols is an ideal guide for scientists who wish to pursue the study of this important branch of cancer research and the development of unique innovative technologies to aid in that study.
Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms. In Expression Profiling in Neuroscience , expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling. The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied. Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments.Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study.
The classification of brain tumors is up-dated using magnetic resonance spectroscopy technology. The role of cellular immortality in brain tumors is reviewed. Tumor to tumor metastases are a common occurrence, for example, , brain metastasis from breast cancer, lung cancer, and renal cancer is discussed. Genetic profiling and treatment (including neurosurgery) of such brain cancers are explained. Breast cancer patients treated with certain drugs (e.g., capecitabine and lapatinib can develop CNS tumors. Role of brain tumor suppressor genes (e.g., NRP/B gene) is pointed out. Biomarkers used to diagnose brain malignancies are explained in detail. A number of imaging modalities used for diagnosing and assessing the effectiveness of treatments of brain tumors are presented. The imaging methods discussed include MRI, PET, CT, MRSi, and SPECT. Also, is discussed the impact of PET using radiolabeled amino acids on brain tumors.
We anticipate the book to be a definitive text on the subject that explores all aspects of the study of adrenal cancer and the treatment of patients with the disease. Chapters will cover epidemiology, pathogenesis, genetics, cancer stem cells, historic and emerging therapies, mouse models of adrenal cancer, new developments in tumor profiling, worldwide collaborative groups and tumor registries together with resources for the practitioner and community of adrenal cancer scientists.We do not wish this book to compete with the other larger books in the Endocrine and Endocrine Surgery literature. In addition, it is not expected to cover benign adrenal diseases that have been covered in detail in other venues. We envision this book to be a very specialized and exhaustive text on basic, translational and clinical aspects of adrenal cancer. This first edition of the textbook Adrenocortical Carcinoma: Basic Sciences and Clinical Concepts provides a concise compilation of relevant data advancing our current understanding of this rare cancer. Chapters are dedicated to various aspects of both the basic biology and the clinical science relating to adrenocortical carcinoma (ACC). Expert clinicians introduce the reader to current state-of-the-art clinical care of patients with ACC. As a considerable body of knowledge on ACC has accumulated over the last decades, expert scientists have authored additional sections that serve as a reference source for the experienced researcher as well as an introduction for the trainee in the laboratory or clinic.Clinical topics span the epidemiology, diagnosis and therapy of ACC. Scientific topics areas span the pathology and pathophysiology of ACC with a special focus on experimental models of adrenocortical tumors and recent advances in the genetic aspects and signaling pathways in ACC. The content of this book was compiled and edited with the intent of facilitating the translation of knowledge into future diagnostic and therapeutic tools that will ultimately improve patient care.